CHAPTER 13 

MEIOSIS AND SEXUAL LIFE CYCLES

 

Introduction

·        Living organisms are distinguished by their ability to reproduce their own kind.

·        Offspring resemble their parents more than they do less closely related individuals of the same species.

·        The transmission of traits from one generation to the next is called heredity or inheritance.

·        However, offspring differ somewhat from parents and siblings, demonstrating variation.

·        Genetics is the study of heredity and variation.

 

A. An Introduction to Heredity

1. Offspring acquire genes from parents by inheriting chromosomes

·        Parents endow their offspring with coded information in the form of genes.

·        Your genome is derived from the thousands of genes that you inherited from your mother and your father.

·        Genes program specific traits that emerge as we develop from fertilized eggs into adults.

·        Your genome may include a gene for freckles, which you inherited from your mother.

·        Genes are segments of DNA.

·        Genetic information is transmitted as specific sequences of the four deoxyribonucleotides in DNA.

·        This is analogous to the symbolic information of letters in which words and sentences are translated into mental images.

·        Cells translate genetic “sentences” into freckles and other features with no resemblance to genes. 

·        Most genes program cells to synthesize specific enzymes and other proteins that produce an organism’s inherited traits.

·        The transmission of hereditary traits has its molecular basis in the precise replication of DNA.

·        This produces copies of genes that can be passed from parents to offspring.

·        In plants and animals, sperm and ova (unfertilized eggs) transmit genes from one generation to the next.

·        After fertilization (fusion) of a sperm cell with an ovum, genes from both parents are present in the nucleus of the fertilized egg.

·        Almost all of the DNA in a eukaryotic cell is subdivided into chromosomes in the nucleus.

·        Tiny amounts of DNA are found in mitochondria and chloroplasts.

·        Every living species has a characteristic number of chromosomes.

·        Humans have 46 in almost all of their cells.

·        Each chromosome consists of a single DNA molecule in association with various proteins.

·        Each chromosome has hundreds or thousands of genes, each at a specific location, its locus.

 

2. Like begets like, more or less: a comparison of asexual and sexual reproduction

·        In asexual reproduction, a single individual passes along copies of all its genes to its offspring.

·        Single-celled eukaryotes reproduce asexually by mitotic cell division to produce two identical daughter cells.

·        Even some multicellular eukaryotes, like hydra, can reproduce by budding cells produced by mitosis.

 

·        Sexual reproduction results in greater variation among offspring than does asexual reproduction.

·        Two parents give rise to offspring that have unique combinations of genes inherited from the parents.

·        Offspring of sexual reproduction vary genetically from their siblings and from both parents.

 

B. The Role of Meiosis in Sexual Life Cycles

·        A life cycle is the generation-to-generation sequence of stages in the reproductive history of an organism.

·        It starts at the conception of an organism and continues until it produces its own offspring.

 

1. Fertilization and meiosis alternate in sexual life cycles

·        In humans, each somatic cell (all cells other than sperm or ovum) has 46 chromosomes.

·        Each chromosome can be distinguished by its size, position of the centromere, and by pattern of staining with certain dyes.

·        A karyotype display of the 46 chromosomes shows 23 pairs of chromosomes, each pair with the same length, centromere position, and staining pattern.

·        These homologous chromosome pairs carry genes that control the same inherited characters.

·        Karyotypes, ordered displays of an individual’s chromosomes, are often prepared with lymphocytes.

·        An exception to the rule of homologous chromosomes is found in the sex chromosomes, the X and the Y.

·        The pattern of inheritance of these chromosomes determines an individual’s sex.

·        Human females have a homologous pair of X chromosomes (XX).

·        Human males have an X and a Y chromosome (XY).

·        Because only small parts of these have the same genes, most of their genes have no counterpart on the other chromosome.

·        The other 22 pairs are called autosomes.

·        The occurrence of homologous pairs of chromosomes is a consequence of sexual reproduction.

·        We inherit one chromosome of each homologous pair from each parent.

·        The 46 chromosomes in a somatic cell can be viewed as two sets of 23, a maternal set and a paternal set.

·        Sperm cells or ova (gametes) have only one set of chromosomes — 22 autosomes and an X or a Y.

·        A cell with a single chromosome set is haploid.

·        For humans, the haploid number of chromosomes is 23 (n = 23).

·        By means of sexual intercourse, a haploid sperm reaches and fuses with a haploid ovum.

·        These cells fuse (syngamy) resulting in fertilization.

·        The fertilized egg (zygote) now has two haploid sets of chromosomes bearing genes from the maternal and paternal family lines.

·        The zygote and all cells with two sets of chromosomes are diploid cells.

·        For humans, the diploid number of chromosomes is 46 (2n = 46).

·        As an organism develops from a zygote to a sexually mature adult, the zygote’s genes are passed on to all somatic cells by mitosis.

·        Gametes, which develop in the gonads, are not produced by mitosis.

·        If gametes were produced by mitosis, the fusion of gametes would produce offspring with four sets of chromosomes after one generation, eight after a second and so on.

·        Instead, gametes undergo the process of meiosis in which the chromosome number is halved.

·        Human sperm or ova have a haploid set of 23 different chromosomes, one from each homologous pair.

·        Fertilization restores the diploid condition by combining two haploid sets of chromosomes.

·        Fertilization and meiosis alternate in sexual life cycles.

·        The timing of meiosis and fertilization does vary among species.

·        The life cycle of humans and other animals is typical of one major type.

·        Gametes, produced by meiosis, are the only haploid cells.

·        Gametes undergo no divisions themselves, but fuse to form a diploid zygote that divides by mitosis to produce a multicellular organism.

·        Most fungi and some protists have a second type of life cycle.

·        The zygote is the only diploid phase.

·        After fusion of two gametes to form a zygote, the zygote undergoes meiosis to produce haploid cells.

·        These haploid cells undergo mitosis to develop into a haploid multicellular adult organism.

·        Some haploid cells develop into gametes by mitosis.

·        Plants and some algae have a third type of life cycle, alternation of generations.

·        This life cycle includes both haploid (gametophyte) and diploid (sporophyte) multicellular stages.

·        Meiosis by the sporophyte produces haploid spores that develop by mitosis into the gametophyte.

·        Gametes produced via mitosis by the gametophyte fuse to form the zygote which produces the sporophyte by mitosis.

 

3. Meiosis reduces chromosome number from diploid to haploid: a closer look

·        Many steps of meiosis resemble steps in mitosis.

·        Both are preceded by the replication of chromosomes.

·        However, in meiosis, there are two consecutive cell divisions, meiosis I and meiosis II, that result in four daughter cells.

·        Each final daughter cell has only half as many chromosomes as the parent cell.

 

·        Meiosis reduces chromosome number by copying the chromosomes once, but dividing twice.

·        The first division, meiosis I, separates homologous chromosomes.

·        The second, meiosis II, separates sister chromatids.

·        Division in meiosis I occurs in four phases: prophase, metaphase, anaphase, and telophase.

·        During the preceding interphase the chromosomes are replicated to form sister chromatids.

·        These are genetically identical and joined at the centromere.

·        Also, the single centrosome is replicated.

·        In prophase I, the chromosomes condense and homologous chromosomes pair up to form tetrads.

·        In a process called synapsis, special proteins attach homologous chromosomes tightly together.

·        At several sites the chromatids of homologous chromosomes are crossed (chiasmata) and segments of the chromosomes are traded.

·        A spindle forms from each centrosome and spindle fibers attached to kinetochores on the chromosomes begin to move the tetrads around.

·        At metaphase I, the tetrads are all arranged at the metaphase plate.

·        Microtubules from one pole are attached to the kinetochore of one chromosome of each tetrad, while those from the other pole are attached to the other.

·        In anaphase I, the homologous chromosomes separate and are pulled toward opposite poles.

·        In telophase I, movement of homologous chromosomes continues until there is a haploid set at each pole.

·        Each chromosome consists of linked sister chromatids.

·        Cytokinesis by the same mechanisms as mitosis usually occurs simultaneously.

·        In some species, nuclei may reform, but there is no further replication of chromosomes.

·        Meiosis II is very similar to mitosis.

·        During prophase II a spindle apparatus forms, attaches to kinetochores of each sister chromatid, and moves them around.

·        Spindle fibers from one pole attach to the kinetochore of one sister chromatid and those of the other pole to the other sister chromatid.

·        At metaphase II, the sister chromatids are arranged at the metaphase plate.

·        The kinetochores of sister chromatids face opposite poles.

·        At anaphase II, the centomeres of sister chromatids separate and the now separate sisters travel toward opposite poles.

·        In telophase II, separated sister chromatids arrive at opposite poles.

·        Nuclei form around the chromatids.

·        Cytokinesis separates the cytoplasm.

·        At the end of meiosis, there are four haploid daughter cells.

·        Mitosis and meiosis have several key differences.

·        The chromosome number is reduced by half in meiosis, but not in mitosis.

·        Mitosis produces daughter cells that are genetically identical to the parent and to each other.

·        Meiosis produces cells that differ from the parent and each other.

·        Three events, unique to meiosis, occur during the first division cycle.

·        1)  During prophase I, homologous chromosomes pair up in a process called synapsis.

·        A protein zipper, the synaptonemal complex, holds homologous chromosomes together tightly.

·        Later in prophase I, the joined homologous chromosomes are visible as a tetrad.

·        At X-shaped regions called chiasmata, sections of nonsister chromatids are exchanged.

·        Chiasmata is the physical manifestation of crossing over, a form of genetic rearrangement.

 

   2) At metaphase I homologous pairs of chromosomes, not individual chromosomes are aligned along the metaphase plate.

·        In humans, you would see 23 tetrads.

   3) At anaphase I, it is homologous chromosomes, not sister chromatids, that separate and are carried to opposite poles of the cell.       

·        Sister chromatids remain attached at the centromere until anaphase II.

·        The processes during the second meiotic division are virtually identical to those of mitosis.

·        Mitosis produces two identical daughter cells, but meiosis produces 4 very different cells.

 

C. Origins of Genetic Variation

1. Sexual life cycles produce genetic variation among offspring

·        The behavior of chromosomes during meiosis and fertilization is responsible for most of the variation that arises each generation during sexual reproduction.

·        Three mechanisms contribute to genetic variation:

   Independent assortment.

   Crossing over.

   Random fertilization.

·        All three mechanisms reshuffle the various genes carried by individual members of a population.

·        Mutations are what ultimately create a population’s diversity of genes.

 

2. Evolutionary adaptation depends on a population’s genetic variation

·        Darwin recognized the importance of genetic variation in evolution via natural selection.

·        A population evolves through the differential reproductive success of its variant members.

·        Those individuals best suited to the local environment leave the most offspring, transmitting their genes in the process.

·        This natural selection results in adaptation, the accumulation of favorable genetic variations.

·        As the environment changes or a population moves to a new environment, new genetic combinations that work best in the new conditions will produce more offspring and these genes will increase.

·        The formerly favored genes will decrease.

·        Sex and mutations are two sources of the continual generation of new genetic variability.

·        Gregor Mendel, a contemporary of Darwin, published a theory of inheritance that helps explain genetic variation.

·        However, this work was largely unknown for over 40 years until 1900.